Page 141 - 006

135

1. 1 67 "6/ ! " buffer phosphate (pH 7.5, 0.1
M˜
67
6/
M) ! " 50 mg buffer 1 ml polyvinul polypyrrolidone (PVPP)

& +/ +/ 71
67 "6/ #( "( ' / "Y

2. ! "'5( ! ( microtube 5 /" ( " &

1
/
1
6/
centrifuge ( "& Y 18,000 # ' (10,000 g) 0 -) 4°C 5 '
3. ((! "'5( 20 µl ! (' potassium phosphate buffer

)
/
/

(pH 7.5, 0.1 M) 1.15 ml 130 µl GSH (glutathione o.o1 M potassium phosphate
buffer 0.1 M, pH 7.5) 10 µl CDNB (1-chlorodinitro-2, 4-benzene, 0.15 M) ("
6

"# '"#
#! J & 20 µl EDTA (ethylene diamine tetraacetic acid,

0.001 M) 1.15 ml potassium phosphate buffer (0.001 M, pH 7.5) 130 µl
GSH 10 µl CDNB
4. 3! ! ( ( "

6/
5. * (& ()(
6 ! ("' ' ( " & spectrophotometer '&
/

+/
0

" & 6/ 340 nm '
30 ' 3 ' & 8 /" &
'
1
,
! H ( (#
' glutathione-S-transferase 5(*
3 - P

monochloronitro-benzene-glutathion (Chotimanothum 2000)

8.4.3 !F # $"$ U $IO Q 8;#E; (Molecular method)

/
0
L
*! #
' ' ! Q
(
+/
0

*

3 (mutation) # gene DNA '& #&
4 & '

/
6
(
' ! Q 5 .4
# (" % DNA markers '* .

1
1

/

6

resistance gene *! #& ' ! Q 5(
*! #

+/
( " L
RFLP (restriction fragment length polymorphisms)
*! #&
/

&
" ( DNA '
(*

(( " 5 * .
1
,
6
(restriction enzyme)

8.4.4 !F # $"$ U $ $I $I (Neurophysiological method)
6/

*
6/ ! ' ! Q (" % & *
6/

/
)
! ' (neurophysiological apparatus) ("
( nerve firing ' * peak

7
+7 !)
' / ' ! Q
*& ' L ' 5( ("
1
1

+ ( " Y 0( # c(3 ( # 3
(+ " - "
6 5
## ! ' "(
6 5 . ' * electrode 5 '3 1 ' /
/

71
7

6/
Y
c

5 % ! ' (nerve ganglia) ( & *! # *
V# * oscilloscope
/

Y

,
+/

Y
! " 5 '* & . * !( 2 ! Q 5 *
V

+7