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154



                                 M





                                                                            71
                                                                                         1



                                 2  
   
(  emulsion    
  !
( 	  "     5   
 
 !   #
  !
(

                                                   6
                                                        /
                   organic extract      
       .% ' partition 
# aqueous phase  (" 8.   "  " '        /
                                                                                                     /


                       alkaline phase solvent '    "  5(  Y
  "  %  benzene, ethyl acetate     diethyl
                                                      71


                                               /


                                                                    /
                                                                           71

                                                                                                     6

                   ether          ' /*  
 ( emulsion   

    solvent '    "  5(  "
     %  hexane
                   petroleum ether
                                !1   	#  L 
   *	( emulsion   *'1 5(  ("
                                1.      !      "  /  	       
 6     !      "  /  	     sodium  sulfate
                                                           71
                                                                    6
                     6 3 +
 sodium sulfate anhydrous *1      Y
   "      "   # K
                                2.       ethanol, isopropyl alcohol, butanol   6  octanol   5  2-3  "(

                                                            /
                                                                 1

                                3.   %  ('   ( '!   ( '   "'     loop  "
 loop   +7    # K
                                                   /
                   %	7  emulsion 1-2   '

                                4.  
    
 ( emulsion   
 
   
 5  emulsion  ("  L  (  L   +/          5

                                                                                           71
                   5( 3     H  "!  !
( (organic extract)     emulsion     
  " "
                  3

                                                                                       	7
                    '              organic  extract    "  # K 2-3  &    	7    "
%  H  *      *

                                                                                             1

                                                                           	7


                   *1              anhydrous sodium sulfate   5   Y
   "
                                                                                              7


                                                              	7
                                                                                                      /
                                5.   %  L  centrifuge   "
   %  emulsion    
5   '& &      L '

                                                                                                     6
                      ! 'L - .  
        *1 
	(              solvent '	7   (5    
5 
   &   !    H
                      (    centrifuge ' /* 5(  	#5(


                        9.6.5    4 "  #	$ #O    	$  8 ‘    # 	2$P  #  	$ UI



                    !8 $	IMR (Effects of co- extractives)
                                Co-extractives ' /
      
 ( M2    
     &    ,!  . 4 
&      2 %  ( &6
                                1.  Artifact  &6 !  '% ( &0 ! #  &   "
#!  '*    &     !  % ( *



                                                                                         ,
                                                                                                   7
                                                                               /


                                                    /
                                                                            6/


                   &   retention time; RT (  "      	7       8(!     5    &    GC *    
9.& (peak)

                                                          /
                                                                             1

                                                                        ,
                                                                          +/

                      !    
  )  
   & "     
   & " 
#!  '*    &       '   
     3 
     &           ,
                                             6
                                                               /
                                                                1

                                        6


                   3(.  (5(      
  " " 3 
     &    
  ' 5( ("'  chemical  derivatization
                                                           ,
                                                                                                     6


                                                                        1
                   GC/MS

                                                                               /


                                                                                      ,
                                2.  Interference !  % (  75    &0 ! #  &   "!  '   &        *  3
                                                                                            +/
                           ,
                      &      K        5(  %  solvent     reagent #   	 *   interference      8(
                                                                                               6/




                                                         6
                            	7




                    &    GC  '     peak  
        * & 0     	 H0 .4#  % (' &   RT    "   5 ( "




                               1

                                                                                /

                      6/



                   3   -	 P,* 
"   % ! ""   (rubber tube)     sodium sulfate anhydrous '5 5(3

                                                                                           /

                                                                       RS
                                                               ,

                                                                  /
                                          +/
                    3 
*   interference    *  3    
     &     !          solvent  #  % (5  3

                        Y





                                      6/
                   
     &     (" &    GC      3     #  
     &      %  '    
(
                               ,

                                                                             ,
                                                                                    1


                   (overlapping) * 
!    (fraction)     chromatographic column   
   cleanup
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