Page 160 - 006
P. 160
154
M
71
1
2
( emulsion
!
( " 5
! #
!
(
6
/
organic extract
.% ' partition
# aqueous phase (" 8. " " ' /
/
alkaline phase solvent ' " 5( Y
" % benzene, ethyl acetate diethyl
71
/
/
71
6
ether ' /*
( emulsion
solvent ' " 5( "
% hexane
petroleum ether
!1 # L
* ( emulsion *'1 5( ("
1. ! " /
6 ! " / sodium sulfate
71
6
6 3 +
sodium sulfate anhydrous *1 Y
" " # K
2. ethanol, isopropyl alcohol, butanol 6 octanol 5 2-3 "(
/
1
3. % (' ( '! ( ' "' loop "
loop +7 # K
/
% 7 emulsion 1-2 '
4.
( emulsion
5 emulsion (" L ( L +/ 5
71
5( 3 H "! !
( (organic extract) emulsion
" "
3
7
' organic extract " # K 2-3 & 7 "
% H * *
1
7
*1 anhydrous sodium sulfate 5 Y
"
7
7
/
5. % L centrifuge "
% emulsion
5 '& & L '
6
! 'L - .
*1
( solvent ' 7 (5
5
& ! H
( centrifuge ' /* 5( #5(
9.6.5 4 " # $ #O $ 8 # 2$P # $ UI
!8 $ IMR (Effects of co- extractives)
Co-extractives ' /
( M2
& ,! . 4
& 2 % ( &6
1. Artifact &6 ! '% ( &0 ! # & "
#! '* & ! % ( *
,
7
/
/
6/
& retention time; RT ( " 7 8(! 5 & GC *
9.& (peak)
/
1
,
+/
!
)
& "
& "
#! '* & '
3
& ,
6
/
1
6
3(. (5(
" " 3
&
' 5( ("' chemical derivatization
,
6
1
GC/MS
/
,
2. Interference ! % ( 75 &0 ! # & "! ' & * 3
+/
,
& K 5( % solvent reagent # * interference 8(
6/
6
7
& GC ' peak
* & 0 H0 .4# % (' & RT " 5 ( "
1
/
6/
3 - P,*
" % ! "" (rubber tube) sodium sulfate anhydrous '5 5(3
/
RS
,
/
+/
3
* interference * 3
& ! solvent # % (5 3
Y
6/
& (" & GC 3 #
& % '
(
,
,
1
(overlapping) *
! (fraction) chromatographic column
cleanup
