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9.4.1 Liquid-Liquid Partitioning M$3 Solvent-Solvent Partitioning
/
,
0
*
& ! #
" ! ' &
#
solvent ! % (
,
0
6/
'1 !
* " 5 solvent ' 7 ! % (
L
H ! & . "
7
RS
! / K (" $ "& ! #
5 " polar non-polar solvent %
0
6/
% acetonitrile £ hexane partitioning ! #
cleanup ! !
( . * ! Q
1
/
/
7
RS
1
0 organochlorine ' 3 ' ! ' " % waxes, pigment fat "
" ") hexane ' /! . 4 "5 ") acetonitrile
9.4.2 Liquid-solid Chromatography (Column cleanup)
7
# &
cleanup % L liquid-liquid partitioning * ! 6
RS
/
" * ' cleanup ( " L liquid-solid chromatography (" %
)
+
1
/
)
chromatography +/ '& &
"
! K ' " ! 3! (" $ "
"
)
,
&
# ! '
* " " V!'5 3!
! V!& V!& ' (stationary
/
)
/
6
/
,
1
0
/
/
phase) V! & 6/ ' (mobile phase) &
# ! ' & ! #
'
()( # V!& ' /
H)
" V! & 6/ ' /' /
(" V!& ' /' / % *
1. alumina ! !1 #
0 organochlorine " ' / 71
/
1
2. alumina ' & 6 #( " silver nitrate %! #
*( V *
!
/
,
RS " %
/1
3. silica gel ! ! #
0 organochlorine, pyrithroid
1
organophosphate #
4. florisil ! ! #
0 carbamate organophosphate
1
#
9.4.3 Gel Permeation Chromatography (GPC)
/
0
"
! (" %& ! H'
'* 5 )
/
0
. stationary phase gels
! ' 2
K * 5 ! H 5 )
0
/
+/
0
)
)
/
0
0
. 5( * H
%
!
! ' ( Y
* 5 . gel
* H
%
' H .4 ("! 2
200-400 ' lipids !
+/
/
)
0
0
71
6
2
600-1500
"
(" L 75
#
"
(" L 7
71
0
column chromatography +/
"
* +7 ")
#
7 ! ' /* & ,
